We are using cookies This website uses cookies in order to offer you the most relevant information. By browsing this website, you accept these cookies.
Mineral deposition was monitored using alizarinred as a vital stain for calcium.
2
Mineralized matrix formation was visualized using alizarinred S staining.
3
Approach and results: Arterial calcium accumulation was evaluated using alizarinred stain and atomic absorption spectrometry.
4
The results of alizarinred staining and ALP detection showed that SpA treatment suppressed the osteogenic differentiation of hBMSCs.
5
Cell counting kit-8 activity was examined to test cell proliferation, and alkaline phosphatase assays and alizarinred S staining were conducted to examine differentiation.
6
Alizarinred staining and ALP activity detection were carried out.
7
Bone formation was assessed by AlizarinRed extraction.
8
AlizarinRed S and Oil Red O staining were used to identify osteoblast and adipocyte differentiation, respectively.
9
Only FK-506 was able to enhance osteocalcin transcripts and AlizarinRed staining, both late markers for differentiation.
10
Flow cytometry, Oil Red O stain and AlizarinRed stain were used to identify the characteristics of HF-MSCs.
11
Alkaline phosphatase (ALP) staining and AlizarinRed staining were used for pathological analysis of the cells.
12
Alizarinred S-positive nodules were first observed on day 14 and then increased in number during the entire culture period.
13
Calcification was verified using Calcein and AlizarinRed S staining or Calcium assays, and molecular analyses showed enhanced expression of calcification-associated genes.
14
Aortic calcification was assessed in 28-day-old mice by measuring the uptake of a fluorescent bisphosphonate probe and by staining tissue sections with Alizarinred.